您是第  994045  位 欢迎访问 中乔新舟官网 ! 全国免费电话:400-038-9959 我的购物车(0) 注 册 / 登 录
您当前的位置:首页 > 产品中心
人基底细胞癌定量PCR芯片试剂盒
英文名:GeneQuery™ Human Basal Cell Carcinoma qPCR Array Kit
货号:GK015
价格:¥4320.00
浏览量:432次
加入购物车,提交订单信息之后,我们会第一时间与您取得联系!
  • 产品说明
  • 产品规格
  • 参考文献
  • 常见问题

人基底细胞癌定量PCR芯片试剂盒描述:


       ScienCell's GeneQuery™ Human Basal Cell Carcinoma qPCR Array Kit (GQH-BCC) is designed to facilitate gene expression profiling of key genes involved in (i) basal cell carcinoma (BCC) biology and carcinogenesis; (ii) targeted BCC therapies; and (iii) hedgehog signaling pathway. 88 genes are selected in this kit based on database and literature research.


       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.


       GeneQuery™ qPCR Array Kit Controls

       Each GeneQuery™ plate contains eight controls (Figure 1).

       Five target housekeeping genes (β-actin, GAPDH, LDHA, NONO, and PPIH), which enable normalization of data.


       The Genomic DNA (gDNA) Control (GDC) detects possible gDNA contamination in the cDNA samples. It contains a primer set targeting a non-transcribed region of the genome.

Positive PCR Control (PPC) tests whether samples contain inhibitors or other factors that may negatively affect gene expression results. The PPC consists of a predispensed synthetic DNA template and a primer set that can amplify it. The sequence of the DNA template is not present in the human genome, and thus tests the efficiency of the polymerase chain reaction itself.


       The No Template Control (NTC) is strongly recommended, and can be used to monitor the DNA contamination introduced during the workflow such as reagents, tips, and the lab bench.


       GeneQuery™ Human Basal Cell Carcinoma qPCR Array Plate Layout* (8 controls in Bold and Italic)


 

1

2

3

4

5

6

7

8

9

10

11

12

A

APC

CD151

CSNK1G2

EIF4E

FZD5

GSK3B

LRP2

RAB23

TCF7L1

WNT11

WNT5B

β-actin

B

APC2

CDH1

CSNK1G3

FAT2

FZD6

HHATL

MC1R

ROR2

TCF7L2

WNT16

WNT7A

GAPDH

C

AXIN1

CEBPA

CTNNB1

FBXW11

FZD7

HHIP

NET1

SHFM1

TGFBR2

WNT2

WNT7B

LDHA

D

AXIN2

CSNK1A1

DHH

FZD1

FZD8

IFNA1

PARP1

SHH

TP53

WNT2B

WNT8A

NONO

E

BMP2

CSNK1A1L

DVL1

FZD10

FZD9

IHH

PRKACA

SMO

TXN

WNT3

WNT8B

PPIH

F

BMP4

CSNK1D

DVL2

FZD2

GAS1

KLF4

PRKACB

STAT2

WNT1

WNT3A

WNT9A

GDC

G

BTRC

CSNK1E

DVL3

FZD3

GLI2

LEF1

PRKACG

SUFU

WNT10A

WNT4

WNT9B

PPC

H

CASP3

CSNK1G1

EGF

FZD4

GLI3

LOC400927

PTCH1

TCF7

WNT10B

WNT5A

ZIC2

NTC


* gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用


货号

GK015

产地

美国

缩写

GQH-BCC

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

参考文献

[1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12.

       [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12.
There are no product questions yet.
公司简介 / Company profile
上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
       公司主要依托复旦大学、同济大学等上海地区多家著名高校,拥有一支富有经验的开发团队,专业从事细胞及细胞...
联系我们 / Contact
电 话:021-56468627;021-56145703
传 真:021-56760351
邮 箱:sales@zqxzbio.com
邮 编:200433
地 址:上海市长江南路180号A区402-406室
Copyright © 2014 ZQXZBIO All rights reserved.

沪公网安备 31011002001038号

技术支持:攸攸网络 沪ICP备14008091号
在线咨询
免费热线
400-038-9959

QQ咨询
我们竭诚为您服务!