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人HIF1信号应答定量PCR芯片试剂盒
英文名:GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit
货号:GK036
价格:¥4320.00
浏览量:425次
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人HIF1信号应答定量PCR芯片试剂盒描述:


       ScienCell's GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation. HIF1 activity is a major component of the human response to hypoxia. Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:


       Cell cycling: DHFR, MAP3K5, POLE2, PRC1, SKP2

       Extracellular matrix remodeling: COL1A1, COL5A1, LOX, P4HA1, PLOD1

       Angiogenesis: HMOX1, MMP2, VEGFA, ANGPTL4, PGF

       Metabolism: PKM, STC2, DHX35, DDX11, ERO1A


       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.


       GeneQuery™ Human HIF1 Signaling Response qPCR Array Plate Layout* (8 controls in Bold and Italic)


 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCB1

BHLHE40

COL18A1

DUSP6

HMOX1

LOXL2

NEK4

PKM

PRC1

RRAS

TSFM

β-actin

B

ADORA2A

BRCA1

COL1A1

ELK1

IGFBP3

MAP3K5

NOTCH4

PLCG2

PSME3

RRM2

TXNIP

GAPDH

C

ADORA2B

CCNE1

COL1A2

ERO1A

INHBA

MCM4

P4HA1

PLOD1

PTGS1

SKP2

UBE2S

LDHA

D

AK3

CCNE2

COL5A1

EXOSC2

INHBE

MEF2A

P4HA2

PLOD2

PTPRB

SMAD9

VEGFA

NONO

E

ANGPTL4

CCNF

CXCR4

FOS

INSR

MLLT10

PAM

POLE2

PTPRR

STC1

VEGFC

PPIH

F

BARD1

CDC6

DDX11

GADD45B

KDM3A

MMP2

PDZD2

POLR3K

RB1

STC2

VLDLR

GDC

G

BCL2

CDK1

DHFR

GINS2

LEPR

MXI1

PELI2

PPARD

RFC5

TFAM

ZNF292

PPC

H

BCL6

CDKN1C

DHX35

HES1

LOX

NDRG1

PGF

PPARG

RNH1

TRIP13

ZWINT

NTC

 

*gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK036

产地

美国

缩写

GQH-HFT

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

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