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人皮炎、哮喘定量PCR芯片试剂盒
英文名:GeneQuery™ Human Dermatitis and Asthma qPCR Array Kit
货号: GK048
价格:¥4320.00
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人皮炎、哮喘定量PCR芯片试剂盒描述:



       ScienCell's GeneQuery™ Human Dermatitis and Asthma qPCR Array Kit (GQH-DER) is designed to facilitate gene expression profiling of 88 key genes involved in the pathogenesis of atopic dermatitis (or eczema) and asthma, which is frequently associated with this chronic inflammatory skin disorder. Genes are also included for related conditions such as allergic contact dermatitis, seborrheic dermatitis, ichthyosis, and eczema herpeticum. Brief examples of how included genes may be grouped according to pathology are shown below:


       Atopic dermatitis: CCL11, IFNG, IL13, IL4, SOCS3, FLG

       Asthma: HNMT, ADRB2, ALOX5, PLA2G7, PHF11

       Allergic contact dermatitis: IL10, IL17A, TNF, CD4, CXCR3

       Seborrheic dermatitis: IDH2, SETBP1, ZNF750, ALOX12B, BTD

       Ichthyosis: LOR, ABC12, KRT1, KRT14, STS

       Eczema herpeticum: TSLP, IRF2, IRF7, IL7R, KLK12


       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.


       GeneQuery™ Human Dermatitis and Asthma qPCR Array Plate Layout* (8 controls in Bold and Italic)


 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCA12

CCL11

CGA

EPX

HNMT

IL18

IL9

LEP

PAFAH1B1

SCGB3A2

TAC1

β-actin

B

ADA

CCL17

COL6A5

FAF2

HRH1

IL2

IRAK3

LOR

PDE4A

SELE

TBX21

GAPDH

C

ADRB2

CCL18

CSF2

FCER1A

ICAM1

IL3

IRF2

LTC4S

PHF11

SETBP1

TGM1

LDHA

D

ALOX12B

CCL22

CXCR3

FCER1G

IDH2

IL31RA

IRF7

MS4A2

PHYH

SLCO2A1

TMEM79

NONO

E

ALOX5

CCL27

CYSLTR1

FCER2

IFNG

IL4

KLK12

NACA

PLA2G7

SOCS3

TNF

PPIH

F

ALOX5AP

CCL5

CYSLTR2

FLG

IL10

IL4R

KLK7

NAT2

PTGDR

SPINK5

TSLP

GDC

G

BCL7B

CD4

DPP10

HAVCR1

IL13

IL5

KRT1

NR3C1

PTGDR2

STAT3

VCAM1

PPC

H

BTD

CD40LG

DSG1

HLA-G

IL17A

IL7R

KRT14

ORMDL3

RNASE3

STS

ZNF750

NTC

  

 * gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK048

产地

美国

缩写

GQH-DER

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

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