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人肝窦内皮细胞

英文名:Human Hepatic Sinusoidal Endothelial Cells
货号:5000
价格:¥15246.00
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  • 参考文献
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人肝窦内皮细胞说明:

       内皮细胞具有多种生理功能,并且是许多病理过程的中心环节。肝脏含有两种不同类型的内皮细胞:血管内皮细胞和肝窦内皮细胞。肝窦内皮细胞是具有类似树突状细胞特殊表型的微血管内皮细胞,并能作为CD4+-T细胞的抗原呈递细胞。因此,肝窦内皮细胞表现为一种新型的器官定植“非专业”抗原呈递细胞,在肝脏局部免疫调控和诱导免疫耐受中发挥作用。肝脏的微环境系统,如门静脉系统成分和来自肝窦细胞的可溶性介质,严密的控制着SEC的抗原呈递作用,以避免出现由免疫介导的损伤。SEC表达特征性的表面受体,并且在形态和代谢上与大血管内皮细胞明显不同。有报道显示在肝再生过程中,SEC是能对环境区域刺激做出快速的局部的反应孔隙的动态调节器。由于SEC在肝窦中的重要位置,使得SEC机能异常和结构改变将会对整个肝脏造成广泛的影响。   

       人肝窦内皮细胞(HHSEC)提取于人肝组织,提取纯化之后立即冻存。每管含有细胞数>5×105 cells/ml,此细胞通过vWF/Factor VIII 和P-CAM (CD31)免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。

配套培养基: 内皮细胞培养基(ECM NO.1001


产品使用说明:仅供科研研究使用.

货号 5000
产地 美国
缩写 HHSEC
规格 5 x 10^5 cells/vial
用途 科研
运输 干冰
保存 液氮
1.) Xie, F., Li, Z.P., Wang, H.W., Fei, X., Jiao, Z.Y., Tang, W.B., Tang, J. & Luo, Y.K. (2016) Evaluation of Liver Ischemia-Reperfusion Injury in Rabbits Using a Nanoscale Ultrasound Contrast Agent Targeting ICAM-1 PLoS One. 11

2.) Soronen, J., Yki?J�rvinen, H., Zhou, Y., S�devirta, S., Sarin, A., Leivonen, M., Sevastianova, K., Perttil�, J., Laurila, P., Sigruener, A., Schmitz, G. & Olkkonen, V.M. (2016) Novel hepatic microRNAs upregulated in human nonalcoholic fatty liver disease Physiol Rep. 4

3.) Koudelkova, P., Weber, G. & Mikulits, W. (2015) Liver Sinusoidal Endothelial Cells Escape Senescence by Loss of p19ARF PLoS One. 10

4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19

5.) Hu, C.T., Guo, L.L., Feng, N., Zhang, L., Zhou, N., Ma, L.L., Shen, L., Tong, G.H., Yan, Q.W., Zhu, S.J., Bian, X.W., Lai, M.D., Deng, Y.J. & Ding, Y.Q. (2015) MIF, secreted by human hepatic sinusoidal endothelial cells, promotes chemotaxis and outgrowth of colorectal cancer in liver prometastasis Oncotarget. 6

6.) Giugliano, S., Kriss, M., Golden-Mason, L., Dobrinskikh, E., Stone, A.E., Soto-Gutierrez, A., Mitchell, A., Khetani, S.R., Yamane, D., Stoddard, M., Li, H., Shaw, G.M., Edwards, M.G., Lemon, S.M., Gale, M., Shah, V.H. & Rosen, H.R. (2015) HCV Infection Induces Autocrine Interferon Signaling by Human Liver Endothelial Cell and Release of Exosomes, Which Inhibits Viral Replication Gastroenterology. 148

7.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2013) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15

8.) Lee SJ, Park SY, Jung MY, Bae SM, Kim IS. (2011) "Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liver." Blood. 117: 5215-23.

9.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2010) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15. 

10.) Guilini C, Urayama K, Turkeri G, Dedeoglu DB, Kurose H, Messaddeq N, Nebigil CG. (2010) "Divergent roles of prokineticin receptors in the endothelial cells: angiogenesis and fenestration." Am J Physiol Heart Circ Physiol. 298: H844-52.

11.) Huebert RC, Vasdev MM, Shergill U, Das A, Huang BQ, Charlton MR, LaRusso NF, Shah VH. (2010) "Aquaporin-1 facilitates angiogenic invasion in the pathological neovasculature that accompanies cirrhosis." Hepatology. 52: 238-48.

12.) Kim Y, Larkin AL, Davis RM, Rajagopalan P. (2010) "The design of in vitro liver sinusoid mimics using chitosan-hyaluronic acid polyelectrolyte multilayers." Tissue Eng Part A. 16: 2731-41. 

13.) Tang L, Yang J, Liu W, Tang X, Chen J, Zhao D, Wang M, Xu F, Lu Y, Liu B, Sun Q, Zhang L, He F. (2009) "Liver sinusoidal endothelial cell lectin, LSECtin, negatively regulates hepatic T-cell immune response." Gastroenterology. 137: 1498-508.e1-5. 

14.) Jung MY, Park SY, Kim IS. (2007) "Stabilin-2 is involved in lymphocyte adhesion to the hepatic sinusoidal endothelium via the interaction with alphaMbeta2 integrin." J Leukoc Biol. 82: 1156-65.

15.) Nahmias Y, Casali M, Barbe L, Berthiaume F, Yarmush ML. (2006) "Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes." Hepatology. 43: 257-65.

16.) Nahmias Y, Odde DJ. (2006) "Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic-endothelial sinusoid-like structures." Nat Protoc. 1: 2288-96.

17.) Perri RE, Langer DA, Chatterjee S, Gibbons SJ, Gadgil J, Cao S, Farrugia G, Shah VH. (2006) "Defects in cGMP-PKG pathway contribute to impaired NO-dependent responses in hepatic stellate cells upon activation." Am J Physiol Gastrointest Liver Physiol. 290: G535-42.


18.)MicroRNA-942 mediates hepatic stellate cell activation by regulating BAMBI expression in human liver fibrosis,Arch Toxicol. 2018 Sep; 92(9): 2935–2946.Published online 2018 Aug 10. doi: 10.1007/s00204-018-2278-9


19.)An oncolytic herpes virus expressing E-cadherin resists NK cell clearance and improves viral spread and glioblastoma virotherapy,Nat Biotechnol. 2018 Nov 26 : 10.1038/nbt.4302.Published online 2018 Nov 26. doi: 10.1038/nbt.4302


Question:
what does the complete medium containing?


Answers:

Posted by Karishma Shah on Tuesday, January 12, 2016
Hello Qinglan,
This product can be used with following Complete media
1001 ECM Endothelial Cell Mediumand it comes with

CAT. NO. CODE DESCRIPTION PRICE

0025 FBS Fetal Bovine Serum, 25 ml
0503 P/S Penicillin/Streptomycin Solution, 5 ml
1052 ECGS Endothelial Cell Growth Supplement
thanks for your interest in ScienCell products
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