人端粒绝对长度和线粒体DNA拷贝数双重定量qPCR检测试剂盒描述:
端粒是染色体末端重复的核苷酸元素,保护染色体免于退化和遗传信息丢失。正常的二倍体细胞在每个细胞周期中都会失去端粒。因此,端粒长度会随着时间的推移而减少,并可能预测寿命。准确和一致的端粒长度定量在细胞生物学的许多方面,如染色体不稳定、DNA修复、衰老、凋亡、细胞功能障碍和肿瘤发生中都很重要。线粒体DNA (mtDNA)是一种环状、多染色体的基因组DNA,位于线粒体中,是一种在能量产生中起关键作用的细胞器。细胞产生能量的能力取决于mtDNA的完整性和拷贝数。有证据表明,端粒长度和线粒体功能障碍在细胞老化和其他年龄相关疾病如癌症、糖尿病和神经退行性疾病中呈正相关。通过PPAR信号通路,两者之间可能存在机制联系。然而,具体的机理还有待阐明。ScienCell's绝对人类端粒长度和线粒体DNA拷贝数双定量qPCR试剂盒(AHDQ)被设计用于同时量化使用qPCR的人类细胞群体的平均端粒长度和mtDNA拷贝数。端粒引物集识别并扩增端粒序列。mtDNA引物集识别并扩增人类mtDNA上最保守的区域之一,不会扩增核基因组DNA上的任何脱靶序列。单拷贝参考(single copy reference, SCR)引物集识别并扩增人类17号染色体上100 bp长的区域,作为数据归一化的参考。已知端粒长度和mtDNA拷贝数的参考基因组DNA样本,作为计算目标样本端粒长度和mtDNA拷贝数的参考。精心设计的引物保证了:(1)高效可靠的定量;(ii)无非特异性扩增。每个引物通过qPCR、熔体曲线分析和凝胶电泳验证扩增特异性,模板序列稀释验证扩增效率。
Kit Components:
Cat # | Component | Quantity | Storage |
---|---|---|---|
MB6018a-1 | 2X GoldNStart TaqGreen qPCR master mix, 1 mL | 3 vials | -20°C |
8958a | Telomere primer set, lyophilized | 1 vial | -20°C |
8958b | Human mtDNA primer set, lyophilized | 1 vial | -20°C |
8958c | Human single copy reference (SCR) primer set, lyophilized | 1 vial | -20°C |
8958d | Nuclease-free H2O | 6 mL | 4 °C |
8958e |
Reference Human genomic DNA sample (Lot #30209, telomere length: 340 ± 18 kb per diploid cell; mtDNA copy number: (1.05 ± 0.05) x 10^3 copies per diploid cell) |
100 μL | -20°C |
货号 |
8958 |
产地 |
美国 |
缩写 |
AHDQ |
规格 |
100 reactions |
用途 |
科研 |
保存 |
液氮 |
运输 |
产品是用干冰装运的。 |
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