产品名称 |
MCF-7人乳腺癌细胞 |
货号 |
ZQ0071 |
产品介绍 |
MCF-7细胞保留了乳腺上皮的分化特性,包括:能通过胞质雌激素受体加工雌二醇;该细胞含有Tx-4癌基因;肿瘤坏死因子α(TNFalpha)可以抑制MCF-7细胞的生长。抗雌激素处理细胞能调节IGFBPs的分泌。 复苏时密度在8.0 x 104 - 1.5 x 105活细胞/cm2之间;传代时接种密度,在4.0 x 104 - 1.0 x 105活细胞/cm2之间。 一般来说,当开始培养MCF-7 时,如果观察到一些悬浮着的细胞或者小的细胞团,在传代时候,需要将这些悬浮的细胞或者小细胞团保留,跟贴壁细胞一起培养, 经过几天的培养后,这些细胞应该会重新贴附在培养瓶上并连接在一起,呈现叠层成岛状(dome)生长。随后,细胞会沿着这些岛边缘,向外生长扩散,在两次传代后,细胞应该会铺展开来,一般6-12天可以达到70-80%的密度。 对于T-25瓶培养, 客户首先按传代密度扩增,制备冻管5支左右,然后开始培养实验用细胞,当传代发现细胞增殖或形态异常时,终止传代,重新复苏细胞。实验用细胞控制传代频率,代数不宜超过5次。 |
种属 |
人 |
性别/年龄 |
女/69岁 |
组织 |
乳腺、胸;分离自转移灶:胸腔积液 |
疾病 |
|
细胞类型 |
|
形态学 |
上皮细胞 |
生长方式 |
贴壁 |
倍增时间 |
|
培养基和添加剂 |
MEM(含NEAA)(中乔新舟 货号:ZQ-300)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%双抗(中乔新舟 货号:CSP006)+1%L-alanyl-L-glutamine(中乔新舟 货号:CSP004)+1%SP(中乔新舟 货号:CSP003)+0.01%human recombinant insulin(中乔新舟 货号:CSP001-10) |
推荐完全培养基货号 |
|
生物安全等级 |
1 |
STR位点信息 |
D5S818: 11,12;D13S317: 11;D7S820: 8,9;D16S539: 11,12;vWA: 14,15;THO1: 6;Amelogenin: X;TPOX: 9,12;CSF1PO: 10 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
|
基因表达 |
|
保藏机构 |
ATCC; CRL-12584 ATCC; HTB-22 BCRC; 60436 BCRJ; 0162 DSMZ; ACC-115 ECACC; 86012803 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0071 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
论文标题: Bacteria eat nanoprobes for aggregation-enhanced imaging and killing diverse microorganisms
DOI: 10.1038/s41467-022-28920-6
发表时间: 2022-03-10
期刊: Nature Communications
影响因子: 14.919
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://www.nature.com/articles/s41467-022-28920-6
论文标题: A metabolic acidity-activatable calcium phosphate probe with fluorescence signal amplification capabilities for non-invasive imaging of tumor malignancy
DOI: 10.1016/j.scib.2021.11.003
发表时间: 2021-11-03
期刊: Science Bulletin
影响因子: 11.78
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S2095927321006952
论文标题: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
DOI: 10.1186/s12951-021-01162-2
发表时间: 2021-12-19
期刊: JOURNAL OF NANOBIOTECHNOLOGY
影响因子: 10.435
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-021-01162-2
论文标题: Prognostic value of gamma-interferon-inducible lysosomal thiol reductase expression in female patients diagnosed with breast cancer
DOI: 10.1002/ijc.33843
发表时间: 2021-10-25
期刊: INTERNATIONAL JOURNAL OF CANCER
影响因子: 7.396
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.33843
论文标题: Anti-tumor potential of astragalus polysaccharides on breast cancer cell line mediated by macrophage activation
DOI: 10.1016/j.msec.2019.01.025
发表时间: 2019-01-08
期刊: Materials Science & Engineering C-Materials for Biological Applications
影响因子: 7.328
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0928493117347409
论文标题: Fabrication and characterization of novel cRGD modified graphene quantum dots for chemo-photothermal combination therapy
DOI: 10.1016/j.snb.2020.127732
发表时间: 2020-01-23
期刊: SENSORS AND ACTUATORS B-CHEMICAL
影响因子: 7.1
货号: ZQ0071
产品名称: MCF-7 (human breast cancer) cell lines
原文链接: https://www.sciencedirect.com/science/article/pii/S0925400520300794
论文标题: Evaluation of anti-tumor effects of crocin on a novel 3D tissue-engineered tumor model based on sodium alginate/gelatin microbead
DOI: 10.1016/j.ijbiomac.2021.01.181
发表时间: 2021-01-30
期刊: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
影响因子: 6.953
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0141813021002294
论文标题: Evaluation of inhibitory effects of geniposide on a tumor model of human breast cancer based on 3D printed Cs/Gel hybrid scaffold
DOI: 10.1016/j.msec.2020.111509
发表时间: 2020-09-11
期刊: Materials Science & Engineering C-Materials for Biological Applications
影响因子: 5.88
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0928493120334275
论文标题: The m6A methyltransferase METTL3 controls epithelial-mesenchymal transition, migration and invasion of breast cancer through the MALAT1/miR-26b/HMGA2 axis
DOI: 10.1186/s12935-021-02113-5
发表时间: 2021-08-21
期刊: Cancer Cell International
影响因子: 5.722
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://link.springer.com/article/10.1186/s12935-021-02113-5
论文标题: Long non-coding RNA ROR recruits histone transmethylase MLL1 to up-regulate TIMP3 expression and promote breast cancer progression
DOI: 10.1186/s12967-020-02682-5
发表时间: 2021-03-02
期刊: Journal of Translational Medicine
影响因子: 5.531
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-020-02682-5
论文标题: MMP-2 sensitive poly(malic acid) micelles stabilized by π–π stacking enable high drug loading capacity
DOI: 10.1039/D0TB01682A
发表时间: 2020-08-11
期刊: Journal of Materials Chemistry B
影响因子: 5.344
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb01682a
论文标题: lncRNA MALAT1 participates in metformin inhibiting the proliferation of breast cancer cell
DOI: 10.1111/jcmm.16742
发表时间: 2021-06-24
期刊: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子: 5.31
货号: ZQ0071
产品名称: MCF7 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16742
论文标题: Construction and evaluation of red blood cells-based drug delivery system for chemo-photothermal therapy
DOI: 10.1016/j.colsurfb.2021.111789
发表时间: 2021-04-26
期刊: COLLOIDS AND SURFACES B-BIOINTERFACES
影响因子: 5.268
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0927776521002332
论文标题: Multiple comparisons of three different sources of biomaterials in the application of tumor tissue engineering in vitro and in vivo
DOI: 10.1016/j.ijbiomac.2019.02.136
发表时间: 2019-02-23
期刊: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
影响因子: 4.784
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0141813019308189
论文标题: Cyclic arginine-glycine-aspartic acid-modified red blood cells for drug delivery: synthesis and in vitro evaluation
DOI: 10.1016/j.jpha.2021.06.003
发表时间: 2021-06-12
期刊: Journal of Pharmaceutical Analysis
影响因子: 4.769
货号: ZQ0071
产品名称: MCF-7 breast cancer cells
原文链接: https://www.sciencedirect.com/science/article/pii/S2095177921000630
论文标题: Multifunctional titanium phosphate carriers for enhancing drug delivery and evaluating real-time therapeutic efficacy of a hydrophobic drug component in Euphorbia kansui
DOI: 10.1039/D1AN00163A
发表时间: 2021-02-05
期刊: ANALYST
影响因子: 4.616
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://pubs.rsc.org/en/content/articlelanding/2021/an/d1an00163a
论文标题: Dual-sensitized Eu(III)/Tb(III) complexes exhibiting tunable luminescence emission and their application in cellular-imaging
DOI: 10.1039/D2DT00051B
发表时间: 2022-01-24
期刊: DALTON TRANSACTIONS
影响因子: 4.569
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://pubs.rsc.org/en/content/articlelanding/2022/dt/d2dt00051b
论文标题: Decellularized Pig Kidney with a Micro-Nano Secondary Structure Contributes to Tumor Progression in 3D Tumor Model
DOI: 10.3390/ma15051935
发表时间: 2022-03-04
期刊: Materials
影响因子: 3.623
货号: ZQ0071
产品名称: Human breast cancer MCF-7 cells
原文链接: https://www.mdpi.com/1529170
论文标题: METTL14 promotes the migration and invasion of breast cancer cells by modulating N6‑methyladenosine and hsa‑miR‑146a‑5p expression
DOI: 10.3892/or.2020.7515
发表时间: 2020-02-24
期刊: ONCOLOGY REPORTS
影响因子: 3.417
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://www.spandidos-publications.com/10.3892/or.2020.7515
论文标题: SYK-targeted dendritic cell-mediated cytotoxic T lymphocytes enhance the effect of immunotherapy on retinoblastoma
DOI: 10.1007/s00432-018-2584-x
发表时间: 2018-01-25
期刊: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
影响因子: 3.282
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://link.springer.com/article/10.1007/s00432-018-2584-x
论文标题: Verteporfin inhibits cell proliferation and induces apoptosis in different subtypes of breast cancer cell lines without light activation
DOI: 10.1186/s12885-020-07555-0
发表时间: 2020-10-29
期刊: BMC CANCER
影响因子: 3.15
货号: ZQ0071
产品名称: MCF-7 cells
原文链接: https://bmccancer.biomedcentral.com/articles/10.1186/s12885-020-07555-0
货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0072 | MDA-MB-453人乳腺癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1800.00 | 放入购物车 》 |
ZQ0077 | ZR-75-1人乳腺癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ0104 | SHZ-88大鼠乳腺癌细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1000.00 | 放入购物车 》 |
ZQ0116 | 人乳腺癌细胞BcaP-37(hela污染,暂不供应) | 5 x 10^5 cells/vial | ¥1200.00 | 放入购物车 》 |
ZQ0118 | MDA-MB-231人乳腺癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ0201 | 4T1小鼠乳腺癌细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ0346 | ZR-75-30人乳腺癌细胞 | 5 x 10^5 cells/vial | ¥1550.00 | 放入购物车 》 |
ZQ0367 | HCC1937人乳腺癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ0372 | Hs 578T人乳腺癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1500.00 | 放入购物车 》 |
ZQ0373 | MDA-MB-468人乳腺癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ505-K | 支原体检测试剂盒 | 1ml/100T | ¥800.00 | 放入购物车 》 |
ZQ506 | 支原体清除试剂盒 | 1ml | ¥600.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
ZQ-300 | MEM(含有NEAA)基础培养基 | 500ml | ¥56.00 | 放入购物车 》 |
ZQ-305 | EMEM(含有NEAA)完全培养基 | 500ml | ¥550.00 | 放入购物车 》 |
ZQ500-A | 优级胎牛血清 | 500ml | ¥2580(开学促销) | 放入购物车 》 |