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人多能干细胞定量PCR芯片试剂盒
英文名:GeneQuery™ Human Pluripotent Stem Cell qPCR Array Kit
货号:GK002
价格:¥4500.00
浏览量:2614次
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  • 产品规格
  • 参考文献
  • 常见问题

人多能干细胞定量PCR芯片试剂盒描述:


       ScienCell's GeneQuery™ human pluripotent stem cell qPCR array kit (GQH-hPSC) is designed to facilitate gene expression profiling of key genes involved in maintaining pluripotency and self-renewal of hPSC, and genes regulated during differentiation of hPSC. 88 genes are selected in this kit based on database and literature research.      


       GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 


       GeneQuery™ Human Pluripotent Stem Cell qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTC1

CDX2

EN2

FOXA2

GFAP

ISL1

MIXL1

NOG

PECAM1

SOX1

TBX6

β-actin

B

AFP

CER1

ENO2

FOXD3

GJA1

KLF4

MYF5

NPM1

POU5F1

SOX2

TCF2

GAPDH

C

ALB

COL1A1

EOMES

FOXJ3

GSC

LEFTYA

MYOD1

NR5A2

PTF1A

SOX7

TCF3

LDHA

D

ALDH1A1

COL2A1

ESG1

GATA2

HAND1

LEFTYB

NANOG

OLIG2

REST

SOX17

TDGF1

NONO

E

BRIX1

CXCR4

ESRRB

GATA4

HDAC2

LIN28

NEUROD

OTX2

REX1

T

TERT

PPIH

F

CD34

DES

FGF4

GATA6

HLXB9

MEF2C

NCAM1

PAX2

RUNX1

TAT

TUBB3

GDC

G

CD90

DNMT3B

FGF5

GBX2

HNF4A

MEIS1

NES

PAX6

RUNX2

TBX3

UTF1

PPC

H

CDH1

EMX2

FLT1

GDF3

IDH1

MESP1

NKX2.5

PDX-1

SALL4

TBX5

VIM

NTC

 

* gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK002

产地

美国

缩写

GQH-hPSC

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

No Publication available at this time

Question:

       1. What things should I add in Positive PCR Control (PPC) ? Customers’ samples or not ? 2. "replicates are strongly recommended (minimum of 3)." Should I buy 3 qPCR Array Kits at least?


Answers:

       Posted by Karishma Shah on Thursday, September 24, 2015

       Hi Sophia, thanks for asking!


       1. PPC control contains a DNA template and a primer set that can amplify it. To verify that qPCR program is working, the 2 components need to add are H2O and qPCR master mix of customer's choice. To be able to detect if there is possible PCR inhibitors in the cDNA samples, cDNA sample, H2O and qPCR master mix should all be added to the PPC well.


       2. Depending on the purpose of the experiments, and the number of samples to compare, the number of kits needed will be based on customer's experiment design.

       Thanks!

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