人MMP-9酶联免疫试剂盒描述:
Matrix metalloproteinase-9 (MMP-9), a 92 kDa type IV collagenase is also known as gelatinase, type V collagenase, or gelatinase B. The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with a molecular mass of 95 kDa. The detected MMP-9 includes zymogen and active enzyme.
ScienCell's human MMP-9 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human MMP-9-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human MMP-9 in the sample that is captured on the strips.
产品使用说明:仅供科研研究使用
货号 |
EK0465 |
产地 |
美国 |
缩写 |
hMMP9-ELISA |
规格 |
96 tests |
用途 |
科研 |
保存 |
频繁使用储存在4°C,很少使用储存在-20°C. |
运输 |
胶冰 |
参考文献 |
1. Collier, I. E.; Bruns, G. A. P.; Goldberg,
G. I.; Gerhard, D. S. On the structure and chromosome location of the 72-and
92-kDa human type IV collagenase genes. Genomics 9: 429-434, 1991. |
2. Turner, H. E.; Nagy, Z.; Esiri, M. M.; Harris, A. L.; Wass, J. A. H. Role of matrix metalloproteinase 9 in pituitary tumor behavior. J. Clin. Endocr. Metab. 85: 2931-2935, 2000.
3. Zhang, B.; Ye, S.; Herrmann, S.-M.; Eriksson, P.; de Maat, M.; Evans, A.; Arveiler, D.; Luc, G.; Cambien, F.; Hamsten, A.; Watkins, H.; Henney, A. M. Functional polymorphism in the regulatory region of gelatinase B gene in relation to severity of coronary atherosclerosis. Circulation 99: 1788-1794, 1999.
4. Coussens, L. M.; Tinkle, C. L.; Hanahan, D.; Werb, Z. MMP-9 supplied by bone marrow-derived cells contributes to skin carcinogenesis. Cell 103: 481-490, 2000.
5. Minematsu, N.; Nakamura, H.; Tateno, H.; Nakajima, T.; Yamaguchi, K. Genetic polymorphism in matrix metalloproteinase-9 and pulmonary emphysema. Biochem. Biophys. Res. Commun. 289: 116-119, 2001.
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