上海中乔新舟生物科技有限公司

猪端粒长度定量定量试剂盒描述：

端粒是染色体末端重复的核苷酸元素，保护染色体免于退化和遗传信息丢失。正常的二倍体细胞在每个细胞周期中都会失去端粒。因此，端粒长度会随着时间的推移而减少，并可能预测寿命。端粒缩短对健康状况有负面影响，并与许多健康问题有关，包括衰老和癌症。准确和一致的端粒长度定量在细胞生物学的许多方面都很重要，如染色体不稳定、DNA修复、衰老、凋亡、细胞功能障碍和肿瘤发生。

ScienCell's绝对猪端粒长度定量qPCR试剂盒(APTLQ)旨在直接测量猪细胞群体的平均端粒长度。端粒引物集识别并扩增端粒序列。单拷贝参考(single copy reference, SCR)引物集识别并扩增了猪13号染色体上100 bp长的区域，作为数据归一化的参考。已知端粒长度的参考基因组DNA样本作为计算目标样本端粒长度的参考。精心设计的引物保证了:(1)高效可靠的定量;(ii)无非特异性扩增。每个引物通过qPCR、熔体曲线分析和凝胶电泳验证扩增特异性，模板序列稀释验证扩增效率。

Kit Components:

Cat #	Component	Quantity	Storage
MB6018a-1	2X GoldNStart TaqGreen qPCR master mix, 1 mL	2 vials	-20°C
8918a	Telomere primer set, lyophilized	1 vial	-20°C
P8918b	Single copy reference (SCR) primer set, lyophilized	1 vial	-20°C
8918c	Nuclease-free H2O	4 mL	4 °C
P8918d	Reference Pig genomic DNA sample (Lot #30500, telomere length: 381 ± 36 kb per diploid cell)	100 μL	-20°C

产品使用说明：仅供科研使用。

货号	P8918
产地	美国
缩写	APTLQ
规格	100 reactions
用途	科研
保存	Please refer to the product sheet
运输	干冰

1.) Martin LF, Richardson LS, da Silva MG, Sheller- Miller S, Menon R. (2019) Dexamethasone Induces Primary Amnion Epithelial Cell Senescence through Telomere-P21 Associated Pathway. Biology of Reproduction.
2.) Bruno S, Sanchez MBH, Pasquino C, Tapparo M, Cedrino M, Tetta C, Camussi G. (2019) "Human Liver-Derived Stem Cells Improve Fibrosis and Inflammation Associated with Nonalcoholic Steatohepatitis." Stem Cells International. 2019
3.) Wysoczanska B, Dratwa M, Gebura K, Mizgala J, Mazur G, Wrobel T, Bogunia-Kubik K. (2019) "Variability Within the Human TERT Gene, Telomere Length and Predisposition to Chronic Lymphocytic Leukemia." OncoTargets and Therapy. 12:4309-4320
4.) Chung SS, Dutta P, Chard N, Wu Y, Chen QH, Chen G, Vadgama J. (2019) "A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-kB inactivation in colorectal cancer cells." Oncotarget. 10(44):4516-4531
5.) Babu H, Ambikan AT, Gabriel EE, Akusjarvi SS, Mupanni NR, Sperk M, Cheedarla N, Palaniapan AN, Sridhar R, Sundaraj V, Tripathy SP, Nowak P, Hanna LE, Neogi U. (2018) Systemic inflammation following long-term successful antiretroviral therapy in people living with HIV (PLHIV). bioRxiv preprint.
6.) Iezzi I, Cerqueni G, Licini c, Lucarini G, Belmonte M. (2018) Dental pulp stem cells senescence and regenerative potential relationship. Journal of Cellular Physiology. 234(5):7186-7197
7.) Lazzarini R, Nicolai M, Pirani V, Mariorri C, DiPrimio R. (2018) Effects of senescent secretory phenotype acquisition on human retinal pigment epithelial stem cells. Aging. 10(11):3173-3184
8.) Shirato K, Takanari J, Koda T, Sakurai T, Ogasawara J, Ohno H, Kizaki T. (2018) A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study. Environmental Health and Preventative Medicine. 23:40

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