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嘌呤霉素10mg/ml; 10 ml
英文名:Puromycin , 10mg/ml; 10 ml
货号:0543
价格:¥3991.00
浏览量:1194次
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  • 产品说明
  • 产品规格
  • 参考文献
  • 常见问题

嘌呤霉素10mg/ml; 10 ml描述:


         Puromycin is an aminonucleoside antibiotic isolated from Streptomyces alboniger. Puromycin terminates protein synthesis at the level of translation in prokaryotic and eukaryotic cells and is active against gram-positive bacteria and weakly active against gram-negative bacteria. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase (pac) gene [1, 2]. Exogenous expression of the pac gene is commonly used in conjunction with puromycin to generate stable cell lines. Most working concentrations range from 1-10 μg/ml; however, the optimal concentration should be determined for individual cell types and culture conditions. In most cases, puromycin kills 99% of non-resistant cells within 48 hours.


产品使用说明:仅供科研研究使用

货号

0543

产地

美国

缩写

PURO-10

规格

10 mg/ml; 10 x 1 ml

用途

科研

包装

管装

保存

- 20° C

运输

干冰

参考文献


[1] Vara J, Perez-Gonzalez JA, Jimenez A. (1985) Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase. Biochemistry 24: 8074-8081. 
[2] Lacalle RA, Pulido D, Vara J, Zalacain M, Jimenez A. (1989) Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger. Gene 79: 375-380.


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