支原体抑制剂描述:
Mycoplasma inhibitor solution is formulated with 10mg/ml ciprofloxacin. Ciprofloxacin belongs to the fluoroquinolone antibiotic family and is effective against mycoplasma, gram positive and gram negative bacteria [1, 2]. Ciprofloxacin inhibits bacterial DNA gyrase necessary for active and non-active growth phases [3].
Add fresh mycoplasma inhibitor solution to culture media and change every 3-4 days. A two week treatment should be sufficient to eliminate contamination. The effective working concentration ranges from 5-25 µg/ml; 10 µg/ml is ideal for most applications. The optimal concentration, however, should be determined for individual cell types and culture conditions.
产品使用说明:仅供科研研究使用
货号 |
0563 |
产地 |
美国 |
缩写 |
MIS |
规格 |
10 mg/ml; 10 x 1 ml |
用途 |
科研 |
包装 |
管装 |
保存 |
- 20° C |
运输 |
干冰 |
参考文献 |
1] Schmitt K, Daubener W, Bitter-Suermann D,
and Hadding U. (1988) "A safe and efficient method for elimination of
cell culture mycoplasmas using ciprofloxacin". J Immunol Methods 109:
17-25. |
[2] Drexler HG and Uphoff CC. (2002) "Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention". Cytotechnology 39: 75-90.
[3] Drlica K and Zhao X. (1997) "DNA gyrase, topoisomerase IV, and the 4-Quinolones". Microbiol Mol Biol Rev 61: 377-392.
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