推荐培养基: （NM, Cat. No. 1521）
1 x 10^6 cells/vial
Typically for cells seeded at 30,000 cells/cm2 in 96-well plate, how many days of culture is required before the cells form neurites and reach ~70% confluency?
The Rat Neurons-hippocampal should start forming neurites after 1 day after thawing the cells. The neurons are non-proliferative cells, so the cells will not proliferate. Cells need to be plated at the density you would like them for your experiments as the cells will not proliferate. Thanks!
Hi, May I ask if I thaw the cells in a tissue culture flask, can I subsequently trypsinize the cells and seed in 96-well plates for my assay? Thanks!
Thanks for asking! We recommend you to plate the Rat Neurons-hippocampal directly in 96-well plate that is cell culture treated so the neurons attach to the plate, rather than plating first in a flask and then moving the cells. These cells do not proliferate, so it is best to seed them onto the 96-well immediately after thawing the cells. Thanks!