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人BMP-4酶联免疫试剂盒

英文名: Human BMP-4 ELISA Kit
货号:EK0314
价格:¥7020.00
浏览量:2689次
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  • 产品说明
  • 产品规格
  • 参考文献
  • 常见问题

人BMP-4酶联免疫试剂盒描述:


       Human bone morphogenetic protein 4 is encoded by the BMP4 gene.It is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. It is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It is involved in muscle development, bone mineralization, and uteric bud development.BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva, in which it is under expressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The BMP4 gene is assigned to the chromosomal location 14q22-q23.The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.


       ScienCell's human BMP-4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human BMP-4 specific-specific monoclonal antibodies are precoated onto 8 x 12 divisible strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added, and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human BMP-4 that is captured in the strips.


产品使用说明:仅供科研研究使用。

货号

EK0314

产地

美国

缩写

hBMP-4-ELISA

规格

96 tests

用途

科研

保存

频繁使用储存在4°C,很少使用储存在-20°C.

运输

胶冰

参考文献

1. Van den Wijngaard A, Weghuis DO, Boersma CJ, van Zoelen EJ, Geurts van Kessel A, Olijve W (Nov 1995). "Fine mapping of the human bone morphogenetic protein-4 gene (BMP4) to chromosome 14q22-q23 by in situ hybridization". Genomics 27 (3): 559-60.
2. Oida S, Iimura T, Maruoka Y, Takeda K, Sasaki S (Nov 1995). "Cloning and sequence of bone morphogenetic protein 4 (BMP-4) from a human placental cDNA library". DNA Seq 5 (5): 273-5

       1. Van den Wijngaard A, Weghuis DO, Boersma CJ, van Zoelen EJ, Geurts van Kessel A, Olijve W (Nov 1995). "Fine mapping of the human bone morphogenetic protein-4 gene (BMP4) to chromosome 14q22-q23 by in situ hybridization". Genomics 27 (3): 559-60.


       2. Oida S, Iimura T, Maruoka Y, Takeda K, Sasaki S (Nov 1995). "Cloning and sequence of bone morphogenetic protein 4 (BMP-4) from a human placental cDNA library". DNA Seq 5 (5): 273-5

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