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CD1小鼠周细胞

英文名:Mouse Brain Vascular Pericytes from CD1
货号:M1200
价格:¥10944.00
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CD1小鼠周细胞

       脑血管周细胞,是血管周细胞,与毛细血管内皮和其它小血管有紧密联系。它位于大脑中内皮细胞和星形胶质细胞之间,通过延长环绕毛细血管的长细胞质与其它细胞相联系。脑血管周细胞参与许多进程,如血管生成,内皮细胞的生存,毛细血管血流量的调节,以及血脑屏障的建立与维持。周皮细胞失调与一些病理学条件有关,如高血压、糖尿病视网膜病变,动脉硬化,多发性硬化,阿茨海默病,以及肿瘤血管发生。脑血管周细胞独特多样的功能使它们成为一个再生医学领域中细胞治疗的新的候选者。脑血管周细胞为理解血脑屏障调节的分子学机制和研究多种中枢神经系统疾病提供了一个有用的体外模型。
    

       ScienCell 实验室的MBVP分离自成熟小鼠的大脑中,一代冻存,冰冻运输。每管细胞密度超过5×105/ml。该细胞经Neural/glial antigen 2 (NG2) 和α-平滑肌肌动蛋白的特异抗体免疫荧光学鉴定。本细胞经检测不含支原体、细菌、酵母菌和真菌。


推荐使用培养液:周边细胞培养基-小鼠(PM-m,NO.1231


产品使用说明:仅供科研研究使用


货号 M1200
产地 美国
缩写 MBVP
规格 5 x 10^5 cells/vial
用途 科研
运输 干冰
保存 液氮

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Question:

Dear Sir, I have isolated pericytes from mouse using pdgfreceptor beta and have maintained them in culture using the pericyte mouse medium from sciencell . I would like to validate the cells for pdgf receptor beta antibody using immunostaining. however the antibody or immunostaining for cells does not seem to work . I saw the pdgf receptor beta antibody immunostaining for mouse brain pericytes done in your cite. I would like to know from you which company (lot no.) pdgf receptor beta antibody is used, corresponding dilution and key steps involved in the staining. The antibodies we have does not seem to work at all for cells but works quite good for tissues. Hence I would be really thankful if you can send across the protocol with antibody details. Thanks Malini

Answers:

  • Posted by Manoj Sharma on Friday, February 12, 2016

    Dear Malini,

    The antibody for PDGF-beta receptor that we use here is from Millipore, Cat. No. is 04-397. The dilution we did is 1:200. But if this is a new antibody for your lab, you may want to try different dilutions for the antibody to find the best concentration for your cells. And we use methanol to fix the cells (fix at room temperature for less than 2 min). 

    SCIENCELL TEAM

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