The 3D Cell Expansion System (3D-CES) is an integrated system designed for large scale expansion of anchorage-dependent cells in a 3-dimensional (3D) microenvironment. Culture and expansion of a large quantity of cells by the traditional two dimensional (2D) method is difficult and known to have many challenges. The 3D-CES is designed to lift this inherent limitation of 2D cell culture and expansion methods. With the 3D-CES, cells are grown and expanded on 3D polystyrene (PS) scaffolds. The unique dynamic system circulates media throughout the system using a peristaltic pump providing an efficient exchange of nutrients and waste between media and the cells. With minimum hands-on time, this innovative technology is more efficient and significantly reduces the amount of time, space, and labor needed to achieve the desired number of cells in a shorter amount of time compared to other methods.
Highlight of the major Features
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Integrated Bioreactor/Incubator System for large-scale cell expansion
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Optimized for Stem Cell (Adipose-Derived & Bone Marrow) expansion
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Cells are seeded and grown on 3D Biotek's polystyrene scaffolds
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No need to change media in the entire expansion process
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Reduced human interaction to minimize chances of contamination
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Each culture chamber is capable of expanding up to 250 million cells in 14-18 days
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Two culture chamber design allows max expansion up to 500 million per production run
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Great potential for use in stem cell therapy and bio-banking
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Also suitable for use in protein production for cosmetic industry
Culture Chamber and Media Container
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The Culture Chamber and Media Container are placed inside a custom-made incubator which provides tubing connection through its side wall to the pump
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Culture Chamber can hold up to 15 pieces of polystyrene (PS) scaffolds
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Cells are seeded and expanded in the PS scaffold which provides a 3D micro-environment
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The capacity of cells for each culture chamber is about 200 - 300 million
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The Media Container can hold up to 3.5 liter
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For 200 - 300 million of cells, about 2.5 liter of media is needed
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Flow of media is from the Media Container in upward direction to the Culture Chamber through the PS scaffolds
Polystyrene (PS) Scaffold
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Fabricated by 3D Biotek's micro-fabrication technology
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Its unique feature is 100% connectivity within its structure which allows effective exchange of nutrients and waste between the media and the cells
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PS scaffold is plasma-treated to enhence cell attachment
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The fiber and pore size are approximately 150 and 200 microns, respectively.
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The dimensions of each scaffold are approximately 105(W)x 66.5(D) x 0.6(H) mm
2-chamber design
It allows a max capacity of about 500 million of cells per single run
This new product can be used to greatly increase the ability to expand various cell types and can play an important role in cell therapy, stem cell therapy, and biobanking.
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