The human leukocyte antigen (HLA) complex is a gene complex located on the short arm of human chromosome 6. The human version of the major histocompatibility complex (MHC) allows the immune system to distinguish the host cells from invaders. It is the most polymorphic locus in the human genome, and has been implicated in many pathologies including autoimmunity disorders and cancer.
ScienCell's GeneQuery™ HLA genotyping kits by Melt Curve Analysis allow for identification of the sample genotypes at a specific genomic location in 90 minutes. Following qPCR with melt curve analysis, the melt temperature (Tm) of the qPCR product is compared to a predetermined threshold temperature (Tthreshold). A Tm higher than Tthresholdsuggests the presence of the target allele, and a Tm lower than Tthreshold suggests the absence of the target allele. For convenience, 2x SYBR® Green qPCR master mix is included in the kits.