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英文名:Astrocyte Medium
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       星形胶质细胞培养基(低血清)包含500 ml基础培养基,10mll胎牛血清(FBS, Cat. No. 0010) 、5ml 星形胶质细胞生长因子(AGS, Cat. No. 1852 )、5 ml青霉素/链霉素溶液(P/S, Cat. No. 0503
货号 1801
产地 美国
缩写 AM
规格 500 ml
用途 科研
包装 瓶装
保存 4℃冰箱
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2.) Herland, A., van der Meer, A.D., FitzGerald, E.A., Park, T.E., Sleeboom, J.J.F. & Ingber, D.E. (2016) Distinct Contributions of Astrocytes and Pericytes to Neuroinflammation Identified in a 3D Human Blood-Brain Barrier on a Chip PLoS One. 11

3.) Zhang, Y., Zhu, T., Zhang, X., Chao, J., Hu, G. & Yao, H. (2015) Role of high-mobility group box 1 in methamphetamine-induced activation and migration of astrocytes J Neuroinflammation. 12

4.) Sreekanthreddy, P., Gromnicova, R., Davies, H., Phillips, J., Romero, I.A. & Male, D. (2015) A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions F1000Res. 4

5.) Rath, B.H., Wahba, A., Camphausen, K. & Tofilon, P.J. (2015) Coculture with astrocytes reduces the radiosensitivity of glioblastoma stem-like cells and identifies additional targets for radiosensitization Cancer Med. 4

6.) Pan, J., Wang, H., Liu, X., Hu, J., Song, W., Luo, J., Jiang, S., Yan, F. & Zhai, B. (2015) Tumor Restrictive Suicide Gene Therapy for Glioma Controlled by the FOS PromoterPLoS One. 10

7.) Lin, J.J., Zhao, T.Z., Cai, W.K., Yang, Y.X., Sun, C., Zhang, Z., Xu, Y.Q., Chang, T. & Li, Z.Y. (2015) Inhibition of histamine receptor 3 suppresses glioblastoma tumor growth, invasion, and epithelial-to-mesenchymal transition Oncotarget. 6

8.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19

9.) Kano, S., Yuan, M., Cardarelli, R.A., Maegawa, G., Higurashi, N., Gaval-Cruz, M., Wilson, A.M., Tristan, C., Kondo, M.A., Chen, Y., Koga, M., Obie, C., Ishizuka, K., Seshadri, S., Srivastava, R., Kato, T.A., Horiuchi, Y., Sedlak, T.W., Lee, Y., Rapoport, J.L., Hirose, S., Okano, H., Valle, D., O'Donnell, P., Sawa, A. & Kai, M. (2015) Clinical utility of neuronal cells directly converted from fibroblasts of patients for neuropsychiatric disorders: studies of lysosomal storage diseases and channelopathy Curr Mol Med. 15

10.) Garwood, C.J., Ratcliffe, L.E., Morgan, S.V., Simpson, J.E., Owens, H., Vazquez-Villase�or, I., Heath, P.R., Romero, I.A., Ince, P.G. & Wharton, S.B. (2015) Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors Mol Brain. 8

11.) Pilakka-Kanthikeel S, Atluri VSR, Sagar V, Saxena SK, Nair M. (2013) "Targeted brain derived neurotropic factors (BDNF) delivery across the blood-brain barrier for neuro-protection using magnetic nano carriers: an in-vitro study." PLoS One. 8: e62241.

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13.) Arscott W, Tandle AT, Zhao S, Shabason JE, Gordon IK, Schlaff CD, Zhang G, Tofilon PJ, Camphausen KA. (2013) 'Ionizing Radiation and Glioblastoma Exosomes: Implications in Tumor Biology and Cell Migration.'

14.) Arscott W, Tandle AT, Zhao S, Shabason JE, Gordon IK, Schlaff CD, Zhang G, Tofilon PJ, Camphausen KA. (2013) "Ionizing Radiation and Glioblastoma Exosomes: Implications in Tumor Biology and Cell Migration." Transloncol. 6: 638-48.

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18.)Proteomic and cellular localisation studies suggest non-tight junction cytoplasmic and nuclear roles for occludin in astrocytes,Eur J Neurosci. 2018 Jun;47(12):1444-1456. doi: 10.1111/ejn.13933. Epub 2018 May 30.

19.)Identification of novel LncRNA targeting Smad2/PKCα signal pathway to negatively regulate malignant progression of glioblastoma,Chuanxi Tang Yue Wang Lei Zhang Jie Wang Wei Wang Xiao Han Chunyan Mu Dianshuai Gao

20.)Interaction of tumor cells and astrocytes promotes breast cancer brain metastases through TGF-β2/ANGPTL4 axes,NPJ Precis Oncol. 2019; 3: 24.Published online 2019 Oct 3. doi: 10.1038/s41698-019-0094-1

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Is it possible to freeze aliquots of complete medium to avoid that growth factors will become unstable at 4°C if the medium stays open longer than 1 month?thanks
Posted by Karishma Shah on Wednesday, September 9, 2015
Hi Silvia,
Thanks for asking!  From our FAQ section: We do not recommend subjecting growth supplements to freeze-thaw, as this can result in reduced activity of components and the potential for components to irreversibly precipitate. Additionally, We do not recommend freezing media, as this can result in irreversible precipitation of media components. Thanks!

Hi, is it recommended to grow HA in 2% FBS (10ml of FBS provided in the kit)? I am working with Astrocytes for the first time and wanted to make sure that I am doing everything right. So basically to make the complete medium, I add 2% FBS, 1% P/S and 1% growth supplement? Thanks!
Posted by Karishma Shah on Monday, August 31, 2015
Hi Harshika,
Thanks for asking! Your process is correct. 
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