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UMNSAH/DF-1鸡胚成纤维细胞(种属鉴定)

英文名:UMNSAH/DF-1
货号:ZQ0169
价格:¥1600.00
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推荐组合

UMNSAH/DF-1鸡胚成纤维细胞(种属鉴定)

¥1600.00
+

UMNSAH/DF-1鸡胚成纤维细胞专用培养基

¥350.00 ¥800.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥1950 ¥2400.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

名称

UMNSAH/DF-1鸡胚成纤维细胞(种属鉴定)

货号

ZQ0169

产品介绍

 UMNSAH/DF-1是起源于10日龄的ELL-0鸡蛋的鸡细胞株,自发永生化。 分离原代鸡胚成纤维细胞并在培养液中培养;传代直到衰老;在衰老过程中离心以保持细胞培养在30%到60%满;不衰老的克隆进行鉴定并传代不少于30次。 在软琼脂上没有观察到克隆增殖,说明这些细胞是永生化而没有转化。 这株细胞可作为病毒增殖、重组蛋白表达和重组病毒生产的基质。  

注意事项:
UMNSAH/DF-1细胞的最佳培养温度也为39℃。而37℃培养会影响细胞状态,建议提前准备专用培养箱。

种属

组织来源

原位;胚胎

形态学

成纤维样

细胞类型

转化细胞系

倍增时间

13.22 hours (Note=In DMEM/F-12 medium), 33.64 hours (Note=In MEM medium), 15.30 hours (Note=In RPMI 1640 medium), 21.39 hours (Note=In DMEM medium) (CelloPub=CLPUB00401)

生长方式

贴壁

培养基和添加剂

DMEM高糖(品牌:中乔新舟 货号:ZQ-100+10%胎牛血清(中乔新舟 货号:ZQ0500+1%双抗(中乔新舟   货号:CSP006

推荐完全培养基货号

ZM0169

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;39

保藏机构

ATCC; CRL-3586

BCRJ; 0307

CCLV; CCLV-RIE 1029

CLS; 305016

IZSLER; BS CL 197

KCB; KCB 2012111YJ

供应限制

仅供科研使用


货号

ZM0169

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


PubMed=9721238; DOI=10.1006/viro.1998.9290
Himly M., Foster D.N., Bottoli I., Iacovoni J.S., Vogt P.K.
The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses.
Virology 248:295-304(1998)

PubMed=9721239; DOI=10.1006/viro.1998.9291
Schaefer-Klein J., Givol I., Barsov E.V., Whitcomb J.M., VanBrocklin M., Foster D.N., Federspiel M.J., Hughes S.H.
The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors.
Virology 248:305-311(1998)

Patent=US6207415
Foster D.N., Foster L.K.
Immortalized cell lines protein production.
Patent number US6207415, 27-Mar-2001

PubMed=11420679; DOI=10.1038/sj.onc.1204378
Kim H., You S., Kim I.-J., Foster L.K., Farris J., Ambady S., Ponce de Leon F.A., Foster D.N.
Alterations in p53 and E2F-1 function common to immortalized chicken embryo fibroblasts.
Oncogene 20:2671-2682(2001)

PubMed=11513975; DOI=10.1016/S0167-4889(01)00131-8
Kim H., You S., Kong B.-W., Foster L.K., Farris J., Foster D.N.
Necrotic cell death by hydrogen peroxide in immortal DF-1 chicken embryo fibroblast cells expressing deregulated MnSOD and catalase.
Biochim. Biophys. Acta 1540:137-146(2001)

PubMed=16313905; DOI=10.1016/j.febslet.2005.10.066
Christman S.A., Kong B.-W., Landry M.M., Kim H., Foster D.N.
Modulation of p53 expression and its role in the conversion to a fully immortalized chicken embryo fibroblast line.
FEBS Lett. 579:6705-6715(2005)

CLPUB00401
Arifin M.A., Mel M., Raus R.A., Sharifah S.H., Ideris A.
Comparison of growth rate and viability of DF-1 cell line in different culture media.
(In) Abstracts of the 2nd international conference on science and technology; pp.1-5; Pulau Pinang (2008)

PubMed=18638503; DOI=10.1016/j.jviromet.2008.06.019
Lee C.-W., Jung K., Jadhao S.J., Suarez D.L.
Evaluation of chicken-origin (DF-1) and quail-origin (QT-6) fibroblast cell lines for replication of avian influenza viruses.
J. Virol. Methods 153:22-28(2008)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=22111699; DOI=10.1186/1471-2164-12-571
Kong B.-W., Lee J.Y., Bottje W.G., Lassiter K., Lee J., Foster D.N.
Genome-wide differential gene expression in immortalized DF-1 chicken embryo fibroblast cell line.
BMC Genomics 12:571.1-571.19(2011)

Patent=US20200179507
Foster D.N., Foster L.K.
Immortalized cell lines and methods of use.
Patent number US20200179507, 11-Jun-2020

PubMed=32005267; DOI=10.1186/s12985-020-1286-6
Pham P.H., Leacy A., Deng L., Nagy E., Susta L.
Isolation of Ontario aquatic bird bornavirus 1 and characterization of its replication in immortalized avian cell lines.
Virol. J. 17:16.1-16.12(2020)

PubMed=33743496; DOI=10.1016/j.psj.2021.101057
Lee J., Kim D.-H., Suh Y., Lee K.
Research note: potential usage of DF-1 cell line as a new cell model for avian adipogenesis.
Poult. Sci. 100:101057.1-101057.4(2021)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)

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