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HT-1376人膀胱癌细胞(STR鉴定)
英文名:HT-1376
货号:ZQ1212
价格:¥1500.00
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推荐组合

HT-1376人膀胱癌细胞(STR鉴定)

¥1500.00
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细胞套餐惊爆价

¥1980
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

HT-1376人膀胱癌细胞

货号

ZQ1212

产品介绍

HT-1376 是从一名 58 岁的白人女性癌症患者膀胱中分离的上皮样细胞。HT-1376细胞系来源于人膀胱癌,特别是三级过渡细胞癌。这条细胞系是由一位有膀胱癌侵袭病史的成年女性患者经尿道切除而得的肿瘤所形成的。HT-1376细胞具有上皮特征,包括微绒毛和索状纤维的存在,这表明它们的上皮起源。此外,这些细胞还显示出若干标记染色体,这些染色体将它们与其他已知的肿瘤细胞系区分开来。HT-1376细胞也是已知的生长在软琼脂和高度致瘤,当注入免疫缺陷的小鼠和仓鼠时,会形成肿瘤。

HT-1376由于其遗传学特征,包括9P21染色体区的显著改变,在膀胱癌研究中具有重要意义。该区域常发生大规模的纯合基因缺失,从而导致CDKN2、CDkn2B和MTAT等重要的抑制肿瘤基因失活。这些缺失在膀胱癌中很常见,对于理解肿瘤发生的分子机制至关重要。例如,CDKN2和CDKN2B的丧失与细胞周期的失调有关,这是癌症进展中的一个关键事件。此外,HT-1376细胞还研究了其表达的P16蛋白,这是CDKN2基因的产物,通常与另一种肿瘤抑制蛋白PRB表达缺失相关。

HT-1376细胞系也被用于病毒学研究,以评估肿瘤病毒的存在,尽管在这些细胞中没有检测到病毒的表达。这使HT-1376成为研究膀胱癌发展和进展的非病毒机制的一个有价值的模型。细胞系的遗传改变及其在体外和体内的生长能力为临床前研究提供了一个坚实的平台,包括药物测试和探索针对膀胱癌特定遗传途径的新治疗策略。

种属

性别/年龄

58岁

组织

膀胱

疾病

膀胱癌

细胞类型

肿瘤细胞系

形态学

上皮细胞样

生长方式

贴壁

倍增时间

~31-60 hours

培养基和添加剂

MEM(含NEAA)培养基(中乔新舟  货号:ZQ-300)  +10%胎牛血清(中乔新舟 货号:ZQ0500+1%P/S(中乔新舟 货号:CSP006

推荐完全培养基货号

ZM1212

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37℃

STR位点信息

Amelogenin  X

CSF1PO  12

D2S1338 17,19

D3S1358 14,17

D5S818  11,12

D7S820  9,12

D8S1179 14,16

D13S317 9,11

D16S539 11,14

D18S51  14,15

D19S433 16

D21S11  26,32.2 (ATCC=CRL-1472; PubMed=11416159)

                26,33.2 (DSMZ=ACC-397)

FGA    21,22

Penta D  11

Penta E  7,13

TH01 7,9.3 (KCLB=21472; PubMed=11416159)

         7,10 (ATCC=CRL-1472; Cosmic-CLP=907066; DSMZ=ACC-397; ECACC=87032402; PubMed=27270441)

TPOX 8

vWA 15,18

抗原表达/受体表达

***

基因表达

***

保藏机构

ATCC; CRL-1472

BCRC; 60058

CLS; 305100

DSMZ; ACC-397

供应限制

仅供科研使用

货号

ZQ1212

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*2支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

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